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. 2023 Jun 22;13:10181. doi: 10.1038/s41598-023-37056-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Titration DELFIAs. This figure shows examples of titration DELFIA graphs for three scFvs from each of the PLA₂, LNTX, and SNTX campaigns. In Supplementary Fig. S2 the titration DELFIAs for all tested scFvs can be observed. Three control antigens, streptavidin and two of the antigens not used in the campaign for the discovery of the given scFv (i.e., antigens used in the SNTX campaign were used as control antigens in the PLA₂ campaign, etc.), were included at the highest concentration (100 nM). In the following descriptions, (CP) and (NCP) refers to antibodies originating from cross-panned or non-cross-panned selectings respectively. (a) TPL0127_02_A02 (CP), (b) TPL_0067_02_E08 (NCP), (c) TPL0229_02_A03 (CP), (d) TPL0127_02_H06 (CP), (e) TPL0066_01_B08 (NCP), (f) TPL0230_02_F07 (CP), (g) TPL0127_01_F09 (CP), (h) TPL0065_02_E02 (CP), and (i) TPL0227_01_E01 (NCP).