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. 2023 Jun 22;13:10181. doi: 10.1038/s41598-023-37056-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of panning strategies. This figure shows the number of scFvs with positive binding signals divided into the different panning strategies. A positive binding signal is determined as a signal value above 2000 after the negative controls have been subtracted. Monoclonal scFvs able to bind to both antigens were only reported as cross-binders and removed from the individual antigen signals. i.e. an scFv binding to antigens A and B was registered as binding to antigen A + B, and not as binding to antigen A and antigen B as well. Panning strategies ending with a hyphen “-“ refers to the second round of panning.