Fig. 1. Expression and Localization of Nat10 enriched in the nucleolus of oocytes in mice.

a Western blot showing the relative expression levels of NAT10 protein among multiple organs in adult WT mice. GAPDH served as a loading control. n = 3 biologically independent samples were included in each group. b Dynamic mRNA expression levels of Nat10 from RNA-seq analyses in oocytes and preimplantation embryos in mice (GSE71434). ICM, Inner cell mass. c Quantitative RT-PCR results showing the relative expression levels of mouse Nat10 mRNA in oocytes, and preimplantation embryos. Data were presented as mean ± SEM, n = 3. GO, growing oocytes collected from postnatal 14-day-old (P14) female mice. d Immunofluorescence (IF) staining of NAT10 in growing (GO), GV, MI, and MII oocytes as indicated. Dashed circle indicates cellular membrane of oocytes. DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar, 20 μm. e IF images of 21-day-old WT ovarian cryosections stained with anti-NAT10 antibody (Red) and DAPI (Blue) for follicles at various stages (primordial, primary, secondary, early antral, and antral stages) as indicated. Scale bar, 20 μm. n = 3 biologically independent samples were included in each group. Boxed inset area is a magnified view of the oocyte in the respective follicles. Arrows point to NAT10-positive nucleus of the oocyte in mouse-developing follicles. f IF images of 21-day-old WT ovarian sections co-stained with NAT10 antibody (Red), Nucleophosmin (NPM, Green), and DAPI (Blue) in the follicles as indicated. Scale bar, 20 μm. n = 3 biologically independent samples were included in each group. Bottom panel is a magnified view of the oocyte in the respective follicles. Arrows point to the co-localization of NAT10 and NPM in the oocyte nucleolus at varied stages of follicles. n = 3 biologically independent samples were included in each group (a–c). Source data are provided as a source data file.