Table 2.
Targets measured in the study.
| Target | Approximate Start date | Approximate End date | Positive control material | Assay reference |
|---|---|---|---|---|
| SARS-CoV-2 N gene | 11/15/20 | 12/31/22 | SARS-CoV-2 gRNA (ATCC VR-1986D) | Wolfe et al.22 |
| SARS-CoV-2 S gene | 11/15/20 | 12/31/22 | SARS-CoV-2 gRNA (ATCC VR-1986D) | Wolfe et al.22 and this paper |
| SARS-CoV-2 ORF1a gene | 11/15/20 | 9/3/21 | SARS-CoV-2 gRNA (ATCC VR-1986D) | Wolfe et al.22 |
| RSV | 1/9/22 | 12/31/22 | Intact RSV B virus (Zepto NATFVP-NNS) | Hughes et al.14 |
| Influenza A | 1/9/22 | 12/31/22 | Twist Synthetic Influenza H3N2 RNA Control (Twist 103002) | CDC33 |
| Wolfe et al.13 | ||||
| Influenza B | 6/6/22 | 12/31/22 | Twist Synthetic Influenza B RNA Control (Twist 103003) | CDC33 |
| Boehm et al.5 | ||||
| Norovirus GII | 11/8/22 | 12/31/22 | Quantitative Synthetic RNA from Norovirus G2 (II) (ATCC VR-3235SD) | Loisy et al.34 |
| Human metapneumovirus | 6/9/22 | 12/31/22 | Gene block | Boehm et al.5 |
| MPOX | 6/18/22 | 12/31/22 | Gene block | Wolfe et al.12 |
| Li et al.35 | ||||
| SARS-CoV-2 HV69-70 | 6/9/22 | 12/31/22 | SARS-CoV-2 variant B.1.1.7, ATCC VR-3326HK | Yu et al.31 |
| Wolfe et al.30 | ||||
| SARS-CoV-2 del156/157 | 9/4/21 | 2/10/22 | Twist Synthetic Delta gRNA control 23 | Yu et al.31 |
| Wolfe et al.30 | ||||
| SARS-CoV-2 del143/145 | 12/6/21 | 2/10/22 | Twist Synthetic Omicron gRNA control 48 | Boehm et al.32 |
| SARS-CoV-2 BA_2_LPPA24S | 2/11/22 | 9/13/22 | Twist synthetic Omicron BA.2 gRNA control 50 | Boehm et al.32 |
| SARS-CoV-2 BA_2_75_S_147E_S_152R | 8/23/22 | 11/15/22 | Gene block | This paper |
| SARS-CoV-2 BA_4_ORF1a_Del_141143 | 5/19/22 | 11/15/22 | Gene block | This paper |
The period of time during which we applied the assay and the positive control material used, as well as the reference for the primers and probes are provided. Approximate start and end dates represent the data of the first and last sample tested using the assay, dates are approximate because some plants may have had a sample before or after the provided date tested, actual first dates for each of the plants is provided in the data file. The vendor Twist Biosciences (“Twist”) is located in South San Francisco, CA. ATCC is American Type Culture Collection.