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. 2023 Jun 22;10:392. doi: 10.1038/s41597-023-02280-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 12 — Particle inspection and filtration by adjusting normalized cross correlation (NCC) score (X axis) and local power (Y axis) for EMPIAR 10017. (A) Intermediate picked particles (green circles) from template-based picking step. The false positives, represented by red crossed particles inside the yellow box, are eliminated in step B. (B) Selected high quality true protein particles through adjustment of NCC and power score values.