Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 22;10:392. doi: 10.1038/s41597-023-02280-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Examples of diverse cryo-EM micrographs in CryoPPP used for particle labelling. (A) Ideal micrographs (EMPIAR-10590) containing protein particles with sufficient contrast that can be easily identified by naked eye. (B) Micrographs containing an abundance of ice patches and contaminations (EMPIAR-10532). Crystalline ice is evident by the cluster of light and dark patches making it difficult to distinguish true protein particles from false positives. (C) Micrograph containing large carbon areas (EMPIAR-10005). (D) Micrographs containing a monodisperse distribution of protein particles (EMPIAR-10852). (E) Large Cluster of clumped protein particles making it difficult to recognize and pick particles (EMPIAR-10387). (F) Micrographs (EMPIAR-11057) containing protein particles overcrowded at upper half region. (G) Difference of ice thickness resulting in the top left part to appear brighter and the bottom right part darker. A crack within the vitrified hole in the lower right part of micrograph causing blurring effect (EMPIAR-10017). (H) Micrograph (EMPIAR-10093) containing heterogeneous top, side, and inclined views of protein particles.