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. 2023 Jun 22;10:392. doi: 10.1038/s41597-023-02280-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The patch-based local and global motion correction pipeline for EMPIAR ID 10737 (E. coli cytochrome bo3 in MSP Nanodiscs). (A) Full frames of micrographs as input. (B) Anisotropic deformation. (C) Rigid motion trajectories plots. Blue: original trajectory, Radish: trajectory with small smoothing penalty, Green: trajectory with fine smoothing. Left: Overall motion trajectory over X and Y motion. Center: X-motion plot over time. Right: Y-motion plot over time. (D) Non-dose weighted aligned averaged micrographs with the highest amount of signal and least amount of motion blur as output.