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. 2023 Jun 22;10:392. doi: 10.1038/s41597-023-02280-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Diagnostic plots of CTF for EMPAIR 10737 (E. coli cytochrome bo3 in MSP Nanodiscs). (A) 1D search over varying defocus values (underfocus). (B) CTF fit plot. X-axis displays frequency, in units in inverse angstroms (Å⁻¹) and Y-axis shows correlation metric between power spectrum (PS) and CTF value. Black: observed experimental power spectrum. Red: calculated CTF. Cyan: cross-correlation (fit).