Table 1.
Strains used in this study
| Strain | Relevant genotype | Parent strain | Source/technique |
|---|---|---|---|
| MG1655 | recF-wt ssb-wt (wild type - wt) | - | (66) |
| EAW629 | ΔrecF::Kan | MG1655 | (52) |
| EAW779 | recF-mKate2::Kan | MG1655 | (52) |
| CJH0015 | recF-mKate2::FRT dnaX-YPet::Kan | MG1655 | (52) |
| EAW1276 | ΔrecF::FRT dnaX-YPet::Kan | EAW629 | This study - P1 JJC5945 |
| EAW1169 | ssb-mTur2::Kan | MG1655 | (67) |
| CJH0080 | ΔrecF::FRT ssb-mTur2::Kan | EAW629 | This study - P1 EAW1169 |
| EAW1130 | PBAD-recF::Kan | MG1655 | This study - λRED |
| EAW1148 | PBAD-recFK36R::Kan | MG1655 | This study - λRED |
| EAW114 | ΔrecO::Kan | MG1655 | (52) |
| EAW1190 | recFK36R::Kan | MG1655 | This study - λRED |
| ZJR04 | Δuup::FRT ΔradD::Kan | MG1655 | (8) |
| EAW1063 | Δuup::FRT ΔradD::FRT ΔrecF::Kan | ZJR04 | (8) |
| EAW1064 | Δuup::FRT ΔradD::FRT ΔrecO::Kan | ZJR04 | (8) |
| CJH0115 | Δuup::FRT ΔradD::FRT recFK36R::Kan | ZJR04 | This study - P1 EAW1190 |
| EAW408 | ΔlacIZYA | MG1655 | (8) |
| EAW1400 | ΔlacIZYA PBAD-recF::Kan | EAW408 | This study - P1 EAW1130 |
| EAW1401 | ΔlacIZYA PBAD-recFK36R::Kan | EAW408 | This study - P1 EAW1148 |
| DH5α | cloning strain | - | (68) |
| CFy7 | Saccharomyces cerevisiae -Yeast strain | (69) | |
| STL2669 | (ΔrecA-srlR)306::Tn10 TetRxonA2 (exoI-) | Susan Lovett gift | |
| BL21 λDE3 ΔslyD | F–ompT hsdSB (rB–, mB–) gal dcm (DE3) ΔslyD | Cox Lab collection |