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. 2023 Apr 18;51(11):5755–5773. doi: 10.1093/nar/gkad272

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — No reduction in eIF4E-mRNA interactions in slf1Δ. (A) Western blots showing eIF4F factor association with eIF4E-TAP in the presence or absence of Slf1. (B) qRT-PCR of mRNAs associated with eIF4E-TAP ± Slf1 ± H₂O₂. ACT1 is in ‘Costello group I’, all other targets are in IIIA. Non-targets: group IVA (COX17, MRP2) or IVC (PRO1) (n = 3). Samples coloured as indicated in boxed key (magenta for WT and cyan for slf1Δ cells, with darker shades for ±H₂O₂). (C) Polysome profiles (A₂₅₄ traces from 15–50% sucrose gradients) of extracts from wild-type (WT) and slf1Δ cells. Underlined areas marked monosome (M) and polysome (P) were pooled for mRNA-seq (n = 3). Right, Ratio of polysome:monosome association for indicated grouped mRNAs in wild-type (WT) and slf1Δ strains. Box plot parameters explained in legend to Figure 1, colours as panel B. P-values shown are *** < 2.2 × 10⁻¹⁶ and ** = 0.00350 (Mann–Whitney test).