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Effect of ssDNA extensions on the RecF ATPase. The ATPase activity of RecF (2.5 μM) was tested in presence of 31mer dsDNA with or without a 5′ or 3′ extension (ext). When indicated, 5 μM of RecR was added to the reactions. The top panel (A) represents the average traces of reactions carried out in triplicate for the ATP hydrolyzed as function of time and the bottom panel (B) provides average rates obtained for each condition with individual values of the triplicate appearing as dots.
