Figure 2. Response of CTPS1- and/or CTPS2-deficient HEK cells to 3-deaza-uridine.
Confluency curves as percentages (%) showing the proliferation of control (Ctr.) or CTPS1- and/or CTPS2-deficient HEK cell lines (CTPS1-KO, CTPS1-low, CTPS2-KO, and CTPS1+2-KO). Confluency measurement using an IncuCyte Zoom system. Cells were seeded for 24 h, and then treated with the indicated concentrations of 3-deaza-uridine (3-DU) in the presence or absence of cytidine or cytidine alone at 200 µM. Data of one representative experiment of three independent experiments.