Figure S4. Inactivation of CTPS1 in Jurkat cells results in rapid cell death and can be counteracted by supplementation of cytidine at 24 h but not at 48 h.

(A, B) Cells were seeded for 24 h, and then cultured in the presence or absence of 200 μM of cytidine or 40 μM of 3-deaza-uridine (3-DU). Apoptotic/cell death markers annexin V and 7-AAD were analysed by flow cytometry at day 3, 5, 6, 7, 10, and 13. Bar graphs from flow cytometry data as presented in Fig 4H showing the percentage (%) of dead, 7-AAD+, alive, and apoptotic cells at 17, 24, 41, 48, 65, 72, 89, and 96 h (h.). (B) Same as (A) except that 200 μM of cytidine has been added at 24 or 48 h. (A, B) Data of one representative experiment of three (A) or two (B) independent experiments.