. 2023 Apr 28;16:100550. doi: 10.1016/j.onehlt.2023.100550

Table 1.

Primers used in this study.

Target gene		Primer sequence (5′-3′)	Amplicon size (bp)	Primer source
ITS 1	Trypanosomes CF	CCGGAAGTTCACCGATATTG	250 to 700	(Njiru et al., 2005)
ITS 1	BR	TTGCTGCGTTCTTCAACGAA	250 to 700	(Njiru et al., 2005)
T. brucei	TBR 1	CGA ATG AAT ATT AAA CAA TGC GCA GT	177 (repetitive)	(Welburn et al., 2001)
T. brucei	TBR 2	AGA ACC ATT TAT TAG CTT TGT TGC	177 (repetitive)	(Welburn et al., 2001)
SRA	SRA A	GACAACAAGTACCTTGGCGC	460	(Gibson et al., 2002)
SRA	SRA E	TACTGTTGTTGTACCGCCGC	460	(Gibson et al., 2002)
SRA	B537	CCATGGCCTTTGACGAAGAGCCCG	743	(Welburn et al., 2001)
	B538	CTCGAGTTTGCTTTTCTGTATTTTTCCC
	Blood meal sources
Vertebrate 16S	Vert 16S F	GAGAAGACCCTRTGGARCTT	250	(Omondi et al.; 2015)
Vertebrate 16S	Vert 16S R	CGCTGTTATCCCTAGGGTA	250	(Omondi et al.; 2015)
Vertebrate cytochrome B	Cyt b F	CCCCTCAGAATGATATTTGTCCTCA	380	(Omondi et al., 2015) (Mejı et al.; 2012) (Boakye et al.; 1999)
	Cyt b R	CATCCAACATCTCAGCATGATGAAA
	Endosymbionts
GPO gene	GPO l F	TGAGAGGTTCGTCAATGA	564	(Dale & Maudlin, 1999)
GPO gene	GPO I R	ACGCTGCGTGACCATTC	564	(Dale & Maudlin, 1999)
Wolbachia surface protein gene	wsp_F1	GTCCAATARSTGATGARGAAAC	714	(Baldo et al., 2006); (Zhou & Neill, 1998)
Wolbachia surface protein gene	wsp_R1	CYGCACCAAYAGYRCTRTAAA	714	(Baldo et al., 2006); (Zhou & Neill, 1998)
Wolbachia 16S rRNA gene	Wspec For	CATACCTATTCGAAGGGATAG	438	(Werren & Windsor, 2000)
Wolbachia 16S rRNA gene	Wspec Rev	AGCTTCGAGTGAAACCAATTC	438	(Werren & Windsor, 2000)
Spiroplasma RPOB	RPOB3044F_ALL	ARTHTTACCADTDGAAGATATGCC	300	(Chepkemoi et al., 2017)
Spiroplasma RPOB	RPOB3380R_ALL	TGTARTTTRTCATCWACCATGTG	300	(Chepkemoi et al., 2017)
SGHV P74 gene	P74 2F	TGTCARATWAATTATCCMCGYGGTAA	373	(Abd-alla et al., 2011)
SGHV P74 gene	P74 2R	AARTCATCGCAATARTAYTTRTT	373	(Abd-alla et al., 2011)