Repeated administration of Δ8-THC induces physical dependence. Mice were treated with Δ8-THC (1-50 mg/kg, s.c.) twice daily for 5 days. On the 6th day, mice received rimonabant (3 mg/kg, i.p.) and were observed for somatic signs of withdrawal: head twitches (A) and paw tremors (B) follow by struggling in the tail suspension test (C). Similarly, mice treated repeatedly with Δ8-THC (50 mg/kg, s.c.) displayed decreased marble burying (D) but no change in mobility (E). Data represent mean±SEM (n = 10[5m/5f]/group);*p<0.05, **p<0.005 v. vehicle.