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. 2023 Jun 9;34(6):1139–1146. doi: 10.1021/acs.bioconjchem.3c00167

Figure 3.

Figure 3

RQ1 DNase-driven assembly of RNA cubes from RNA/DNA fibers and their immunostimulation assessed in reporter cell lines. (a) Schematics of DNase-driven transition for RNA/DNA fibers to RNA cubes. The proposed mechanism assumes two-steps: degradation of dsDNA toeholds, followed by digestion of hybrid duplexes and cubes assembly. (b) Confirmation of RNA/DNA fiber assembly by the agarose gel. (c) Stepwise degradation of RNA/DNA fibers by RQ1 DNase and assembly of RNA cubes confirmed by native-PAGE. (d) AFM images of RNA/DNA fibers and resulting RNA cubes. (e) Scheme of TLR7 activation with subsequent expression and secretion of SEAP to cell media. Normalized fold induction of TLR7 by individual treatments to untreated cells (n = 4, ± SEM). (f) Scheme of RIG-I activation with subsequent expression of Lucia luciferase that is secreted to cell media and normalized fold induction of RIG-I by individual treatments to untreated cells (n = 3, ± SEM). (e, f) Statistical analysis by ordinary one-way ANOVA (p < 0.05).