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. 2023 Apr 7;3(3):270–282. doi: 10.1021/acsbiomedchemau.2c00048

Figure 1.

Figure 1

Effect of zafirlukast analogues on TNF-induced NF-κB activation. FACS data demonstrate surface expression of TNFR1 (A) and TNFR2 (B) on HEK293 cells. Cells were incubated with the anti-TNFR1 or TNFR2 antibody and followed by the AlexaFluor647-conjugated secondary antibody. Luciferase assay of NF-κB activation in HEK293 cells transfected with luciferase reporter plasmids and treated with 0.5 μM (C, E) and 5 μM (D, F) zafirlukast analogues for 2 h, followed by the addition of TNF (10 ng/mL) for 24 h. Data are means ± SD from three experiments.