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Specificity of zafirlukast analogues. (A) Effect of zafirlukast analogues on TNF-induced RelB activation (TNFR2 noncanonical pathway). HUVEC cells transfected with membrane TNF for 6 h and then treated with zafirlukast analogues (250 nM), and then analyzed with Western blot. (B) Quantification of RelB protein bands by densitometry using ImageJ.
