Fig. 1.

Single-cell transcriptomic profiling of young and aged mouse brains after ischemic stroke. (A) Experimental design for single-cell RNA transcriptomic profiling of young and aged mouse brains after ischemic stroke. tMCAO or sham surgical operations were performed on young and aged mice. Ischemic left hemispheres were harvested at d3 and d14 after tMCAO, and left hemispheres were also collected from sham mice at d3 after tMCAO. (B) UMAP visualization of a total of 32,446 cells from all 11 nonhemorrhagic sample hemispheres (including sham), clustered into 18 cell types based on core markers shown in Dataset S1. (C) Violin plots showing logarithmic-scale expression levels of prototypic markers for each color-coded cell type after clustering was performed in (B). (D) UMAP visualization showing the color-coded major subsets after subclustering of Pecam1⁺ ECs and Olig1⁺ oligodendrocyte lineage cells, including OPCs and OLs. See markers for each subclusters in SI Appendix, Fig.S3 and Dataset S2.