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. 2023 Jun 12;120(25):e2300374120. doi: 10.1073/pnas.2300374120

Fig. 5.

Fig. 5.

Modifying IGF1 signaling is sufficient to scale both tooth size and cusp patterning. (A) Igf1 is up-regulated in the rat molars (n = 5 for all stages) compared to mouse molars (n = 7 per stage except late cap stage n = 6). (B) IGF1-treated molars are larger (n = 9, P = 0.0339) and have more spread of secondary enamel knots ex vivo (n = 9, P = 0.0001) than those of the controls (n = 11 for both measurements). (C) Igf1r KO mouse molars are smaller (E18, n = 6, P < 0.0045) and their secondary enamel knots are less spread (n = 6, P < 0.0055) than those of wild-type mouse molars (E17, n = 4). (D) Wild-type and Igf1r KO mouse molars at E19 when all the main cusps are visible. Boxes enclose 50% of observations, the horizontal bar denotes the median, and whiskers extend to last values within 1.5 interquartiles. Anterior to the left, buccal to the top. (Scale bars, 200 μm.)