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. 2023 Jun 13;120(25):e2304055120. doi: 10.1073/pnas.2304055120

Fig. 4.

Fig. 4.

Open MHC-I improves peptide exchange efficiency on a broad repertoire of HLA allotypes. (A and B) Schematic summary of fitted kinetics obtained from FP analyses of peptide exchange, showing (A) the dissociation of 40 nM TAMRATAX9/A02 in the presence of 1 μM unlabeled TAX9 and (B) 40 nM TAMRATAX9 in different concentrations of TAX8/A02 (50, 75, 100, and 200 nM). (C) The dissociation profiles of 40 nM WT or open TAMRATAX9/A02 in the presence of 1 μM unlabeled TAX9, and association profiles of 40 nM WT or open TAMRATAX9 in 50 nM TAX8/A02, as indicated. (D) Linear correlations between the apparent rate constants Kassoc. and the concentrations of TAX8/A02. The extrapolation of the slope between Kassoc. and the concentrations of TAX8/A02 determine the apparent association rate kon. (E) Log-scale comparison of Kassoc. for the WT (black) or the open (pink) HLA-A*02:01, A*01:01, A*24:02, A*29:02, A*30:01, B*07:02, B*08:01, B*15:01, B*37:01, B*38:01, B*58:01, E*01:03, and G*01:01. The apparent rate constant Kassoc. was determined by fitting the raw trace to a monoexponential association model. NA indicates no fitted Kassoc.. Results of three technical replicates (mean ± σ) are plotted.