Fig. 4.

Targeting IGF2BP2 mitigates migration, invasion, and angiogenesis in LUAD cells. A Selection of the optimal siRNAs targeting IGF2BP2 through RT-qPCR. B, C Verification of IGF2BP2 mRNA expression in A549 and NCI-H1299 cells after transfection with si-NC or si-IGF2BP2. D-F Wound healing scratches at 0 h, 24 h, and 48 h for A549 and NCI-H1299 cells transfected with si-NC or si-IGF2BP2. Bar, 200 μm. G, H Transwell assay for detecting invasive A549 and NCI-H1299 cells after transfection with si-NC or si-IGF2BP2. Bar, 50 μm. I-K Images of HUVECs cultured with conditioned medium from A549 cells transfected with si-NC or si-IGF2BP2, and quantification of branch points and capillary length. Bar, 200 μm. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001