Fig. 1.

METTL3 shows decreased expression in endometriosis specimens and primary ESCs. A The mRNA m6A levels in human ectopic with strict match eutopic endometium, and normal control endometrium of 30 paired specimens were detected by ELISA via an m6A RNA methylation colorimetric quantification kit. B The global m6A level in mRNA in human ectopic, eutopic, and normal control endometrium measured by m6A dot blot assay with anti-m6A antibody. C The mRNA level adjusted to GAPDH of methyltransferases, demethylases and N6-methyladenosine readers in EMs and paired tissues by RT-qPCR. D Protein level of METTL3 in EMs and paired specimens by western blotting, using GAPDH as an internal control. E Representative images illuminate METTL3 expression in EMs and paired tissues by immunohistochemistry (IHC) (scale bars = 100 μm and 50 μm). Relative expression of METTL3 in primary Ectopic(ESCs), Eutopic(EuSCs), and Normal control(NESCs) endometrium cells in mRNA level by RT-qPCR (F) and protein level by western blotting (G), using GAPDH as an internal control. All above result were shown in means ± SD, ns, p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001