Table 1.
Twelve in silico predicted significant mutations characteristic of the Beijing 14717-15-cluster
| Gene | AA exchange | PAM1 | SIFT P | Product | Function, category (https://mycobrowser.epfl.ch/) |
|---|---|---|---|---|---|
| Rv0360c | Trp89STOP | 0 | - | Conserved protein | Conserved hypotheticals. Function unknown |
| Rv1866 | Asp699Tyr | 0 | 0.01 | Conserved protein | Lipid metabolism. Function unknown, but supposed involvement in lipid degradation. |
| Rv1661 (pks7) | Leu2076Arg | 1 | 0.01 | Probable polyketide synthase Pks7 | Lipid metabolism. Potentially involved in some intermediate steps for synthesis of polyketide molecule which may be involved in secondary metabolism |
| Rv1154c | Met(s)57Ile | 2 | 0.00 | Hypothetical protein | Conserved hypotheticals. Function unknown |
| Rv0567 | Ser76Cys | 5 | 0.02 | Probable methyltransferase/methylase | Intermediary metabolism and respiration. Causes methylation. |
| Rv0064 | Ala225Pro | 13 | 0.01 | Probable conserved transmembrane protein | Cell wall and cell processes. |
| Rv2263 | Gly25Ser | 16 | 0.00 | Possible oxidoreductase | Intermediary metabolism and respiration. Oxidoreduction. |
| Rv3350c (PPE56) | Gly2482Ser | 16 | 0.00 | PPE family protein | Function unknown |
| Rv1077 (cbs) | Val77Ala | 18 | 0.02 | Probable cystathionine beta-synthase | Intermediary metabolism and respiration. Thought to be involved in homocysteine transulfuration. |
| Rv0823c | Thr218Met(s) | 32 | 0.00 | Possible transcriptional regulatory protein | Regulatory proteins. Thought to be involved in transcriptional mechanism. |
| Rv0152c (PE2) | Asp217Asn | 36 | 0.02 | PE family protein | Function unknown |
| Rv2607 (pdxH) | Asp84Asn | 36 | 0.04 | Probable pyridoxamine 5’-phosphate oxidase | Intermediary metabolism and respiration. Involved in biosynthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. |
Substitution is predicted to affect protein function if SIFT P < 0.05. Database UniProt + TrEMBL was used SIFT analysis