Figure 2.

Donor MABs form myotubes in vitro. Spontaneous differentiation of donor MABs obtained from the medicinal product before infusion. 2x10⁵ MABs were plated on low-growth factor matrigel coated 3.5 cm Petri dish, in proliferation medium (Megacell). After an O/N incubation at 37°C, 5% CO₂, proliferation medium was replaced by differentiation medium (DMEM supplemented with 2% horse serum), and differentiation extended for 10 days. Cells were stained with anti-myosin heavy chain antibody and Dapi. Reprinted from (204).