Figure 4.

Role of oligomerization for IB formation. (a) Schematic depiction of EBOV NP and NP mutants with point mutations in amino acids necessary for oligomerization. IDRs and known interaction sites with other viral proteins are shown. (b) Expression of EBOV NP and an oligomerization-deficient NP mutant. Vinculin was used as a loading control. (c) Immunofluorescence imaging of NP or an oligomerization-deficient NP mutant (transfected amounts as indicated) expressed in cells in presence or absence of VP35. As a negative control the NP expression plasmid was omitted. Representative images out of three independent experiments with at least two images each per sample are shown. Scale bar = 30 µm.