Figure 5. Apoptotic effect of 7-hydroxy-3,4-dihydrocadalene.

MCF7 cells were treated with IC₅₀ and IC₅₀/2 and H₂O₂ 1.0 mM as positive control for 48 h. (A) Caspase 3 and caspase 9 activities were measured using the fluorimetric substrate Ac-DEVD-AMC. (B) The cell lysates were analyzed by Western blot for the indicated cleaved-caspase 3, reprobed for Bcl-2 and GAPDH was used as a loading control. Results are shown as means ± SD of three independent experiments (**P < 0.01 and ****P < 0.0001 vs. control; ^####P < 0.0001 vs. hydrogen peroxide and ⁺⁺⁺P < 0.001 vs. IC₅₀/2).