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. 2023 Jun 8;14(12):2320–2332. doi: 10.1021/acschemneuro.3c00069

Figure 8.

Figure 8

HPC modulates DNA methylation of the BDNF gene to upregulate its expression in mice. (A) The results from qPCR indicated that HPC upregulated BDNF mRNA expression in the hippocampus of mice, and 5-Aza treatment increased BDNF in control groups. (B) The results from western blot indicated that HPC enhanced BDNF protein expression in the hippocampus of mice, and 5-Aza treatment augmented BDNF in control groups. (C) Representative confocal images of BDNF immunoreactivity in the hippocampus of different groups. BDNF was stained green. The nucleus was stained with DAPI and manifested as blue. The scale bar is 50 μm. (D) Fluorescence intensity of BDNF in different groups. (E) Correlation coefficients provided for the association between BDNF expression and DNA methylation. The data are presented as mean ± SEM, n = 6 for each group. Compared with the control group: *P < 0.05. Compared with the hypoxia group: #P < 0.05. Compared with the HPC group: &P < 0.05.