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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res (Hoboken). 2023 Apr 26;47(6):1067–1078. doi: 10.1111/acer.15082

Figure 1. Experimental design.

Figure 1.

Participants underwent two neurobehavioral experimental sessions initiated at 1:00 pm on each day, one week apart. During each session, an intravenous line for repeated measurement of blood cortisol levels was inserted 45 minutes prior to the session (−45 timepoint), followed by a pre-scan ATT assessment of initial alcohol self-motivation conducted with alcoholic beer (Alcohol) or non-alcoholic (Placebo) beer for 10 minutes. This was immediately followed by three, 5-minute blocks of Arterial Spin Label (ASL) perfusion MRI scanning procedure, for a total of 15 minutes ASL measurement and total time in the scanner of 55 minutes. Immediately post MRI scans, participants underwent a second, post-scan ATT with non-alcoholic beer in both sessions to assess sustained alcohol motivation. Measurements of breath alcohol level (BrAC), cortisol, subjective craving, stimulation and sedation were obtained before and after the pre-Scan ATT (−10, 0), during scanning (+10, +25, +35, +45) and at post-Scan ATT(+60, +70) timepoints.