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Construction and confirmation of the VHH library. (A) PCR amplification of the vh sequence. (B) PCR amplification of vhh sequences. (C) Insertion rate of vhh into pADL-10b. Sixteen colonies were randomly selected, and vhh sequences were amplified by PCR. (D) Diversity of the VHH library. Amplified vhh sequences were sequenced, and the FR sequences and CDR sequences of VHHs are shown.
