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. 2023 Jun 21;64(7):32. doi: 10.1167/iovs.64.7.32

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Figure 6. — HNF4A impairs angiogenesis in PDR by regulating the CACNA1A/VEGFA axis in vitro. (A) Expression of HNF4A, CACNA1A, and VEGFA in HRMECs treated with sh-HNF4A, sh-VEGFA, and oe-CACNA1A either separately or in combination determined by qPCR. (B) Expression of HNF4A, CACNA1A, and VEGFA in HRMECs treated with sh-HNF4A, sh-VEGFA, and oe-CACNA1A either separately or in combination determined by Western blot. (C) Tube-forming ability of HRMECs treated with sh-HNF4A, sh-VEGFA, and oe-CACNA1A either separately or in combination. */^# P < 0.05, compared with HRMECs treated with sh-NC + oe-NC + sh-NC. The cell experiment was run in triplicate independently.