Figure 8. L. plantarum (Lp) inhibits the expression of the growth repressor fezzik in a general control nonderepressible 2 (GCN2)-dependent manner.

(A) Expression of fezzik in germ-free (GF) larvae (grey) or larvae associated with Lp WT (dark green), Lp:Tn_r/tRNA (black), or Lp:Tn_control (light green) in a control background (Mex>mCherry^RNAi) or in larvae knocked down for GCN2 (Mex>GCN2^RNAi) or ATF4 (Mex>ATF4^RNAi) in enterocytes. The data is from the RNAseq described in Figure 7. (B) Developmental timing of GF larvae (grey) or larvae associated with Lp WT larvae (green) or LpΔop_r/tRNA in a control background (Mex>mCherry^RNAi, filled circles) or fezzik knock-down larvae (Mex>fezzik^RNAi, empty circles). The graph represents the total fraction of emerged pupae over time as a percentage of the final number of pupae. The graphs show five replicates per condition (mean and standard deviation). Each replicate consists in one tube containing 40 larvae. Larvae were reared on imbalanced diet (FLY AA –60% Val). We used a Cox proportional hazards model to compare the effect of fezzik knock-down in Lp, LpΔop_r/tRNA, and GF larvae. For each microbial condition, we used a post hoc Tukey test to compare the fezzik knock-down to control. ***: p-value <0.001.

Figure 8—figure supplement 1. fezzik RNAi efficiently represses the expression of fezzik in enterocytes.

Expression of fezzik in the anterior midgut of germ-free (GF) larvae on imbalanced diet (FLY AA –60% Val) in a control background (Mex>mCherryRNAi, filled circles) or in a fezzik knock-down in enterocytes (Mex>fizz^RNAi). Expression is normalised with the expression of rp49 using the formula 2^{Cq(rp49)-Cq(fezzik)}. We performed a Mann-Whitney test. *p-value <0.05.