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. 2023 Jun 23;14:107. doi: 10.1007/s12672-023-00715-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — HSF1 affects oxidative stress and lipid peroxidation levels by regulating HSPE1 in vitro. A, B CKK-8 assay after HSF1 knockout (*P < 0.05). C, D Mitochondrial membrane potential assay in PC3 cell line via JC-1 staining and flow cytometry (*P < 0.05). E–G ROS and MDA assays in PC3 cell lines after HSF1 knockout (*P < 0.05). H–J ROS and MDA assays in LNCaP cell lines after HSF1 knockout. K, M ROS and MDA assays in PC3 cell lines after HSPE1 overexpression (*P < 0.05)