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. 2023 Jun 23;6:664. doi: 10.1038/s42003-023-05043-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Relative mRNA expression of nuclear-basket NUPs, comparing the progenitor-state versus the differentiated (day 4) primary human keratinocytes, based on RNA-seq data (multiple unpaired t test, N = 3 biological replicates). b Western blots showing the distribution of nuclear-basket NUPs in the fractions from size-exclusion chromatography (SEC), using the soluble extraction from the nuclei of progenitor-state keratinocytes. The fractions corresponding to the protein standards for SEC are labeled on the top. c, d Western blots showing the co-immunoprecipitation between NUP98 and RAE1 in the soluble extraction from the nuclei (progenitor-state keratinocytes). NUP98 and RAE1 co-immunoprecipitated each other, but not other nuclear-pore subunits such as TPR. e RT-qPCR comparing the relative NUP98 expression at the mRNA level in the progenitor-state, early- (day2) and mid- (day4) differentiation state of keratinocytes (one-way ANOVA with post-hoc test, N = 3 biological replicates, data are represented as mean ± standard deviation). f, g Western blots and quantifications comparing NUP98 protein expression in keratinocyte differentiation, with Lamin A/C used as the loading control (One-way ANOVA with post-hoc test, N = 4, data are represented as mean ± standard deviation). h RT-qPCR comparing the relative RAE1 mRNA expression in keratinocyte differentiation (one-way ANOVA with post-hoc test, data are represented as mean ± standard deviation). i, j Western blots and quantifications comparing RAE1 protein expression levels in keratinocyte differentiation, with Lamin A/C used as the loading control (one-way ANOVA with post-hoc test, N = 3, quantification data are represented as mean ± standard deviation).