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. 2023 Jun 23;13:10247. doi: 10.1038/s41598-023-37187-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental flowchart for MCAO modeling. The success rate of filament insertion = Animals with filament successfully inserted into the skull and blocked the MCA/animals involved in filament insertion; Postoperative mortality rate = Model animals that died after surgery (animals that died during surgery are not included)/model animals with successful insertion filament; Subarachnoid hemorrhage rate = Model animals with postoperative subarachnoid hemorrhage/model animals with successful insertion filament; MCAO model success rate = Successful model animals/animals involved in surgical modeling (successful model animal must meet one of the following characteristics: (1) TTC staining or MRI arise a significant infarct, or (2) neurological defect score (Zea Longa score) is 1 to 3 marks, and did not sacrifice during the test period).