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. 2023 Jun 23;14:3761. doi: 10.1038/s41467-023-39291-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The pancreas of Nfic^-/- mice has a reduced relative size (n = 3/group, two-tailed Student T-Test, P = 0.0013). B Principal component analysis of the RNA-Seq transcriptomes from WT and Nfic^-/- mice. C RT-qPCR showing reduced expression of transcripts coding for digestive enzymes and pancreatic TF in Nfic^-/- pancreata [n = 3/group; P < 0.1 (#), P < 0.05 (*), P < 0.01 (**), two-tailed Mann Whitney U-Test. Nfic, P = 0.0015; Amy2a5, P = 0.0006; Cela, P = 0.011; Cpa, P = 0.009; Ctrb1, P = 0.034; Cela1, P = 0.2187; Cela2, P = 0.024; Cela3, P = 0.028; Pnlip, P = 0.003; Rnase1, P = 0.25; Spink3, P = 0.035; Ptf1a, P = 0.015; Rbpjl, P = 0.73; Nr5a2, P = 0.015; Nr0b2, P = 0.035]. D Western blotting showing reduced expression of digestive enzymes, but not NR5A2 (P = 0.654), in Nfic^-/- pancreata (n = 7/group). E IF analysis of the expression of PTF1A in pancreatic epithelial CDH1+ cells of Nfic WT and KO mice. (Scale bar = 10 μm). Fluorescence quantification shown in the accompanying bar graph (P = 0.03, two-tailed Student T-test). Densitometric quantification of panel 3B: band intensity normalized to loading control, relative to wild-type pancreata (n = 5/group). F RT-qPCR showing reduced expression of transcripts coding for digestive enzymes in primary acini from Nfic^-/- mice [n = 5/group, P < 0.1 (#), P < 0.05 (*), P < 0.01 (**); two-tailed Mann–Whitney U-test. Nfic, P = 0.008; Amy2b, P = 0.015; Ctrb1, P = 0.007; Cpa, P = 0.095]. G Comparison of the overlap of DEG in the pancreas of Nfic^-/- vs. that of mice lacking NR5A2, PTF1A, and MIST1 (details in text). Statistics: two-tailed Student T-test. Significant overlap is shown for downregulated genes compared to a random list of genes. “N-1” chi-squared test was used to calculate statistical significance. Barplots are presented as mean values +/− SD. Boxplots show the minimum, the maximum, the sample median, and the first and third quartiles. Source data are provided as a Source Data file.