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. 2023 Jun 23;14:3761. doi: 10.1038/s41467-023-39291-x

Fig. 6. NFIC is dynamically regulated during acute caerulein pancreatitis and is required for a homeostatic recovery.

Fig. 6

A RNA-Seq analysis of Nfic, Nr5a2, and Ptf1a expression in wild-type mice upon induction of a mild acute pancreatitis (n = 3/group). Significance was calculated compared to expression at 0 h (P < 0.05 (*) (T-test analogical method; Nfic at 8 h, P = 0.014; at 24 h, P = 0.45; at 48 h, P = 0.54; Nr5a2 at 8 h, P = 0.023; at 24 h, P = 0.033; at 48 h, P = 0.036; Ptf1a at 8 h, P = 0.012; at 24 h, P = 0.027; at 48 h, P = 0.034). B IF analysis of NFIC and PTF1A upon pancreatitis induction showing NFIC down-regulation (n = 4/group, scale bar = 20μm). C Quantification of PTF1A+ and NFIC+ cells in wild-type mice during pancreatitis. D Histological analysis of wild-type and Nfic-/- pancreata 48 h and 5 days after the induction of pancreatitis showing increased damage in mutant mice (n ≥ 4/condition, scale bar = 20 μm). E Pancreatitis scoring shows impaired recovery of Nfic-/- mice at 48 h and day 5 (n ≥ 4/condition). Damage was scored as (0–3) for each parameter analyzed (n = 4/group). F, G IHC reveals increased expression of KRT19, a higher number of KI67+ acinar cells, and increased infiltration by CD45+ cells in Nfic-/- pancreata. Representative images are shown in (F), (scale bar = 10 μm); quantification of CD45, KRT19 and Ki67 expression (G) as described in Methods [n = 5/ group; P < 0.1 (#), P < 0.05 (*), P < 0.01 (**); two-tailed Mann–Whitney U-test. CD45: at 24 h, P = 1; at 48 h, P = 0.061; at day 5, P = 0.008). KRT19: at 48 h, P = 0.021; at day 5, P = 0.049. KI-67: at 24 h, P = 0.009; at 48 h, P = 0.038; at day 14, P = 0.059]. H RT-qPCR expression analysis showing up-regulation of Ddit3 and Hsd17b11 in Nfic-/- pancreata in basal conditions and after induction of an acute pancreatitis (n = 4 mice/group). Chop: at 0 h, P = 0.034; at 48 h, P = 0.046; at day 5, P = 0.057; Hsd17b11: at 0 h, P = 0.028; at 48 h, P = 0.057; at day 5, P = 0.028. Data are presented as mean values +/− SD. P < 0.05 (*). Source data are provided as a Source Data file.