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. 2023 Jun 23;13:10221. doi: 10.1038/s41598-023-34795-4

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© The Author(s) 2023

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Splice sites are four conserved nucleotides: the donor sequence GU at the 5′ and the acceptor sequence AG at the 3′ end of an intron. After the splicing process pre-mRNA is converted to mature mRNA.