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. 2023 Jun 23;13:10241. doi: 10.1038/s41598-023-37116-x

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Size distribution of the lobe dimensions and the full molecule length. (a) Model of holo-transferrin indicating the different sizes (long lobe dimension, short lobe dimension, full molecule length) evaluated here. Since each molecule’s orientation determines whether the longer or the shorter lobe dimension is approximately aligned with the full-length axis, we use long and short dimension here rather than length and width. (b) LEEH amplitude reconstruction of an individual holo-transferrin molecule and cross sections for long lobe dimension, short lobe dimension and full molecule length as indicated by the green, blue and yellow lines. (c) Experimentally obtained size distributions of long lobe dimension, short lobe dimension, and full molecule length for holo-transferrin fitted with Gaussians. The mean values and standard deviations of the distributions are given in Table 1. (d)–(f) Corresponding data for apo-transferrin.