Fig. 2.

Alignments of amino acid sequences for MHC-I exon 3 and MHC-IIB exon 2 of common buzzards (Buteo buteo). a) Alignment of Buteo buteo MHC-I exon 3 putative alleles include sequences from human (accession # L06425) and white-tailed eagle (Haliaeetus albicilla, accession # MK186004), with numbering of residues 92–179 based on HLA-A2 alignment. b) Alignment of Buteo buteo MHC-IIB exon 2 putative alleles include sequences from human (accession # NP_002115) and Galapagos hawk (Buteo galapagoensis, accession # EU876825), with numbering of residues 9–93 based on HLA-DRB1 alignment. Dots indicate amino acids identical to the top sequence of MHC-I exon 3 and MHC-IIB exon 2, respectively. Circles represent human peptide binding residues, triangles represent inferred non-passerine peptide binding residues, and squares represent chicken peptide binding residues. PPS: pervasive positive selection, EPS: episodic positive selection, PNS: pervasive negative selection