| Antibodies |
|
|
|
| APC anti-mouse CD117 (c-kit) Recombinant Antibody |
BioLegend |
155107 |
| APC anti-human FcεRIα Antibody |
BioLegend |
334612 |
| BV711 Mouse Anti-Human CD117 |
BD Biosciences |
740831 |
| BB700 Mouse Anti-Human FcεR1α |
BD Biosciences |
747780 |
| BUV395 Mouse Anti-Human CD282 (TLR2) |
BD Biosciences |
742772 |
| Texas Red™ Avidin D |
Vector Laboratories |
A-2006-5 |
| FITC anti-human CD44 Antibody |
BioLegend |
397517 |
| PE anti-mouse CD117 (c-Kit) Antibody |
BioLegend |
105807 |
| APC anti-human MRGX2 Antibody |
BioLegend |
359005 |
| APC anti-human CD324 (E-Cadherin) Antibody |
BioLegend |
324107 |
| FITC anti-human CD45 |
BioLegend |
982316 |
| APC anti-human CD31 Antibody |
BioLegend |
303115 |
| FITC anti-human CD36 Antibody |
BioLegend |
336203 |
| FITC anti-human CD39 Antibody |
BioLegend |
328205 |
| APC anti-human CD90 (Thy1) Antibody |
BioLegend |
328113 |
| FITC anti-human CD282 (TLR2) Antibody |
BioLegend |
392307 |
| CD284 (TLR4) Monoclonal Antibody (HTA125), eBioscience |
ThermoFisher |
14-9917-82 |
| TLR2 Recombinant Rabbit Monoclonal Antibody (JM22-41) |
ThermoFisher |
MA5-32787 |
| PE anti-mouse CD282 (TLR2) Antibody |
BioLegend |
148603 |
| Anti-Mast Cell Chymase antibody |
Abcam |
Ab233103 |
| DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate) |
BioLegend |
422801 |
| Recombinant Anti-TLR2 antibody [EPR20302-119] |
Abcam |
Ab209216 |
|
| Bacterial and virus strains |
|
|
|
|
Staphylococcus epidermidis(SE) ATCC12228 |
Gallo lab (UCSD) |
N/A |
|
Staphylococcus aureus SA113 Rosenbach
|
Gallo lab (UCSD) |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Recombinant Human SCF Protein |
R&D Systems |
255-SC-200/CF |
| Recombinant Human IL-6 Protein |
R&D Systems |
206-IL-200/CF |
| Recombinant Human IL-3 Protein |
R&D Systems |
203-IL-100/CF |
| HyClone Characterized Fetal Bovine Serum (FBS), U.S. Origin |
HyClone |
SH30071.03 |
| HEPES (1 M) |
ThermoFisher |
15630080 |
| L-Glutamine (200 mM) |
ThermoFisher |
25030081 |
| Sodium Pyruvate (100 mM) |
ThermoFisher |
11360070 |
| RPMI 1640 Medium |
Invitrogen |
11-875-085 |
| Antibiotic-Antimycotic (100X) |
ThermoFisher |
15240096 |
| PBS, pH 7.4 |
ThermoFisher |
10010023 |
| Bovine Serum Albumin (BSA) Powder–Serum Replacement Grade |
GeminiBio |
SKU# 700-100P-100 |
| Trypan Blue Solution, 0.4% |
ThermoFisher |
15250061 |
| Dead Cell Removal Kit |
Miltenyi |
130-090-101 |
| RNeasy Mini Kit (250) |
Qiagen |
74106 |
| iScript™ cDNA Synthesis Kit, 100 × 20 μL rxns #1708891 |
Bio-Rad |
1708890 |
| Biorad real-time PCR system |
Bio-Rad |
N/A |
| Collagenase, Type II |
Worthington |
LS004176 |
| Collagenase, Type IV, powder |
Gibco |
17104019 |
| Dnase |
Roche |
SKU 4716728001 |
| Lipopolysaccharide (LPS) |
Invivogen |
14D9 |
| Human CD117/c-kit DuoSet ELISA |
R&D Biosystems |
DY332 |
| Human CCL2/MCP-1 DuoSet ELISA |
R&D Biosystems |
DY279 |
| Human GM-CSF DuoSet ELISA |
R&D Biosystems |
DY215 |
| Human IL-8/CXCL8 DuoSet ELISA |
R&D Biosystems |
DY208 |
| Toluidine blue |
Sigma- Aldrich |
89640-5G |
| Stemline® II Hematopoietic Stem Cell Expansion Medium |
Sigma-Aldrich |
S0192 |
| Hyaluronan (High MW) 100MG |
Sigma-Aldrich |
GLR002 |
| Hyaluronan (Low MW) 100MG |
Sigma-Aldrich |
GLR001 |
| Hyaluronan DuoSet ELISA |
R&D Biosystems |
DY3614-05 |
| Lipoteichoic acid from S. aureus |
InvivoGen |
tlrl-slta |
|
| Deposited data |
|
|
|
| RNA seq triplicate with commensal bacteria |
GSE223180 |
NCBI NIH |
| RNA seq duplicate for LTA |
GSE223180 |
NCBI NIH |
| Sc RNA seq Dermal fibroblasts and Mast cells |
GSE223180 |
NCBI NIH |
|
| Experimental models: Cell lines |
|
|
|
| Primary Dermal Fibroblast; Normal, Human, Adult (HDFa) |
American Type culture collection (ATCC) |
PCS-201-012 |
| Human ex-vivo skin |
GenoSkin |
order.accessusa@genoskin.com
|
| Normal CD34+ Cells |
Astarte |
1014-759AP11 |
| CD34+ cells from human cord blood |
UCSD |
IRB: Project #190445X |
|
| Experimental models: Organisms/strains |
|
|
|
| B6.Cg-KitW-sh/HNihrJaeBsmJ (KitW-sh) |
Jackson Lab |
Strain #012861; RRID:IMSR_JAX:012861 |
| NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) |
Jackson Lab |
Strain #005557; RRID:IMSR_JAX:005557 |
|
| Oligonucleotides |
|
|
|
| TRAF1 TaqMan™ Gene Expression Assay (FAM) |
Applied Biosystems |
4453320 |
| TNFAIP3 |
Applied Biosystems |
4453320 |
| NFKBIA |
Applied Biosystems |
4453320 |
| IL-8 (CXCL8) |
Applied Biosystems |
4453320 |
| TLR2 |
Applied Biosystems |
4453320 |
| TLR4 |
Applied Biosystems |
4453320 |
| GAPDH |
Applied Biosystems |
4453320 |
|
| Software and algorithms |
|
|
|
| Partek Flow Build version 10.0.22.0330 |
Partek |
https://www.partek.com/
|
| FlowJo v.10.5.3 |
TreeStar |
FlowJo LLC Oregon |
| GraphPad Prism 9.4.1 |
GraphPad |
GraphPad Prism 9.4.1 (458) |
| Pipeline Version: cellranger-6.0.0 |
Loupe cell browser |
10X genomics |
| R analysis, alignment-free quantification was performed via Kallisto of transcripts. Transcript expression converted via tximport in R. Differential gene expression (DE) in R was calculated via Sleuth or EDGER. |
N/A |
R Statistical Software (v4.1.2; R Core Team 2021) |
|
| Other |
|
|
|
| Millipore Guava EasyCyte 8HT Flow Cytometer |
Millipore |
12568 |
| Fluorescence microscope |
Zeiss |
N/A |
| Reichert hemocytometer |
Hausser scientific |
02-671-5 |
| Spectra Max ID3 |
Molecular devices |
N/A |
| Luminex 200 RUO System w/xPONENT 4.3 |
Luminex |
LX200-XPON-RUO |
| MAGPIX System |
Luminex |
MAGPIX System |
| FLEXMAP 3D with xPONENT 4.2 |
Luminex |
FLEXMAP-3D-RUO |
