Fig. 5. Myosin VI is required for efficient localization of WRNIP1 to stalled forks.

a, b Models of how myosin VI could mediate fork protection in its role as a motor protein. c Myosin VI depletion interferes with efficient fork localization of WRNIP1. U2OS cells were siRNA-transfected, followed by SIRF assays as indicated. Left: dot plots of PLA signal intensities with mean values −/+ 95% confidence intervals. Significance levels were calculated using the two-tailed Mann–Whitney test from indicated number of nuclei per sample (ns, non-significant, ****: p < 0.0001, ***: p < 0.001, *: p < 0.05) and annotated for p > 0.0001. Right: representative images, Hoechst (blue), PLA (magenta), scale bar = 10 µm. A representative experiment from three independent replicates is shown. Knockdown efficiency is shown in Supplementary Fig. 6. Source data are provided as a Source Data file.