Fig. 6. Analysis of PPIX-interacting proteins identifies NIH3T3-specific cluster of PPIX-binding proteins related to iron and mitochondrial metabolism.

a Weighted gene co-expression network analysis (WGCNA) of the PPB and tandem mass tag (TMT) quantitative proteomics data identified a cluster of proteins enriched in NIH3T3 cells. b Highest candidate PPIX-binding proteins from NIH-3T3 cluster 1 shown relative to other cell lines (n = 2 biologically independent samples, mean). c STRING protein-protein interaction (PPI) network analysis of cluster 1 identifies a protein module enriched for iron and oxidative phosphorylation. d Enrichr analysis of cluster 1 identifies porphyria and iron relationships. e PPIX-binding proteins identified in screen (orange) demonstrating a role in iron pathway and ferroptosis.