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. 2023 Jun 24;14:3777. doi: 10.1038/s41467-023-39105-0

Fig. 3. Detection of siRNA cleavage by p19-T111BpyAla using fluorescence polarization assay.

Fig. 3

a The overall hydrolytic RNA cleavage mechanism due to the incorporation of BpyAla into p19. b An overall schematic representation of the experimental strategy, in which Cy3-Gl2 siRNA cleavage was assessed using fluorescence polarization. Created with BioRender.com. c Fluorescence polarization assay was used to visualize the cleaving potential of p19-T111BpyAla. The assay was conducted using 5 µM p19-T111BpyAla or p19-WT and 200 nM RNA, n = 3, in which n values represent 3 independent replicates. Red dots represent p19-T111BpyAla, and black dots represent p19-WT.