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Optimal lipid formulations of mRNA vaccines via a high-throughput ionizable lipidoid screening system. (A) Schematic illustrating a three-dimensional combinatorial synthesis library for lipidoids. (B) Optimal lipidoids selection using mRNA transduction efficiency screening (C) Structures and candidates of heterocyclic amine combined lipidoids. (D) Schematic diagram of mRNA vaccine LNP-containing with heterocyclic lipid. Adapted with permission from reference [128].
