Table 1.
Co-IP/MS results for O1 as bait protein and 3 related kinesin-like proteins
| Accession | Name | Antibody 1 | Antibody 2 | ||
|---|---|---|---|---|---|
| WT peptides | o1 peptides | WT peptides | o1 peptides | ||
| Zm00001d052110_P042 | OPAQUE1 | 546/435/546 | 0/0/0 | 474/493/458 | 0/0/0 |
| Zm00001d052110_P007 | OPAQUE1 | 258/229/268 | 0/0/0 | 230/232/213 | 0/0/0 |
| Zm00001d022276_P012 | KINESIN-LIKE PROTEIN 12C | 29/3/18 | 0/0/0 | 36/56/8 | 1/0/0 |
| Zm00001d041353_P002 | KINESIN-LIKE PROTEIN 12D | 12/6/9 | 0/0/0 | 16/24/5 | 0/0/0 |
| Zm00001d034030_P017 | KINESIN-LIKE PROTEIN 12E | 3/3/3 | 0/0/0 | 7/9/6 | 4/2/0 |
Co-IPs were performed using two independently generated antibodies against O1. For each antibody, 3 biological replicates were conducted using wild-type plants and o1-N1242A mutants as a negative control. The number of peptides identified for each replicate are separated by a slash. At least 2 isoforms of O1 are present in developing leaves; no peptide corresponding to O1 was identified in homozygous o1 mutants. The POK-like kinesins KIN12C and KIN12D were found only in wild-type samples and are considered plausible interactors. Fewer peptides were found for the related KIN12E and did not meet the threshold for a probable high-confidence interactor.