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. 2023 Jun 12;14:1170940. doi: 10.3389/fgene.2023.1170940

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Copyright © 2023 Nousiainen, Eloranta, Isoaho, Cairo, Wilson, Heikinheimo and Pihlajoki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of UBE2C and its effect on HUH6 and HB-243 cell viability. Following the siRNA transfection, UBE2C mRNA expression in HUH6 was reduced by 95% (A) and protein expression by 80% (B) compared to cells transfected with non-targeting (NT) siRNA. In HB-243, UBE2C mRNA expression was reduced by 98% (D) and protein expression by 80% (E). WST-1 assay showed a 24% decrease in HUH6 (C) and 44% in HB-243 (F) in cell viability after UBE2C silencing. Bar plots are presented as relative values of mean of three independent experiments ± RSD. **p-value <0.01, NT = non-targeting. Normalization factor (NF) describing the amount of total protein in lane in relation to other lanes is given beneath the bands (B, E).