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. 2023 Jun 12;11:1066788. doi: 10.3389/fcell.2023.1066788

FIGURE 2.

FIGURE 2

TRPV4 activation promotes osteogenic differentiation and affects mitochondrial energetic and respiration. (A). ALP analysis of MC3T3-E1 cells (7 Days) in presence of increasing concentration of TRPV4 activator 4αPDD (B). ALP analysis by using TRPV4 inhibitor RN1734 in MC3T3-E1 cells (C). ALP analysis of RCO (2 Days) in presence of increasing concentration of TRPV4 activator 4αPDD (D). ALP assay was carried out by using TRPV4 inhibitor RN1734 (E). ALP analysis of RCO (7 Days) in presence of increasing concentration of TRPV4 activator 4αPDD. (* = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001, one-way ANOVA) (F). TRPV4 activation leads to increased nodule formation as assessed by Alizarin Red-S assay (G–I). TRPV4 activation leads to increased expression of osteogenic genes (BMP2, RUNX2, COL1) (J). TRPV4 activation leads to increased mitochondrial parameters. Average values of biological repeats (n = 3) are shown. The values are Student’s t-test, ns: non-significant, * = p< 0.05, ** = p< 0.01, **** = p < 0.0001.