Figure 6.

Plots of viral titers for CHIKV and MAYV in WT and transgenic mosquitoes. To evaluate the replication of CHIKV and MAYV, we measured viral titers infection (virus present in midgut tissues), dissemination (virus present in legs/wings tissues), and transmission (virus present in saliva samples). Female mosquitoes 5–7 days old were fed virus-spiked bloodmeals using artificial membrane feeders, and only fully engorged mosquitoes were separated into new containers. Seven days post-infection, mosquitoes were cold-anesthetized and individual samples were collected. Viral titers were measured via plaque assay on Vero cells 7 dpi. Data points of the 40 replicates obtained in two independent experiments are depicted on the plot. All undetected mosquito samples were given a value of half the limit of detection (LOD/2) for statistical analyses. Red horizontal bars represent the mean with SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Kruskal-Wallis test with Dunn’s correction for multiple comparisons. Infectious blood meal (IBM) titers: CHIKV = 5.63E+07, MAYV = 3.63E+07.