Table 3.

Vector competence of transgenic and WT Ae. aegypti mosquitoes for CHIKV H-20235 and MAYV 12A.

Virus	Transgenic line	Infection (midgut)	Dissemination (legs/wings)	Transmission (saliva)
CHIKV H-20235 IBM titer: 5.63E+07	AE1	39/40 (97.5%) 1.0000	35/40 (87.5%) 0.0547	7/40 (17.5%) <0.0001
	AE5	38/40 (95.0%) 0.4937	32/40 (80.0%) 0.0053	10/40 (25.0%) <0.0001
	WT	40/40 (100%) N/A	40/40 (100%) N/A	33/40 (82.5%) N/A
MAYV 12A IBM titer: 3.63E+07	AE1	29/40 (72.5%) 0.0004	23/40 (57.5%) 0.0005	16/40 (40.0%) 0.0243
	AE5	28/40 (70.0%) 0.0002	28/40 (70.0%) 0.0198	12/40 (30%) 0.0016
	WT	40/40 (100%) N/A	37/40 (92.5%) N/A	27/40 (67.5%) N/A

Bold formatting indicates statistical significance. Data are presented as the number of virus-positive/number tested with rates of infection (virus present in midgut tissues), dissemination (virus present in legs/wings tissues), and transmission (virus present in saliva samples) indicated by percentages in parentheses. Two-tailed p values of infection, dissemination, and transmission (compared to WT) indicated in italics. Statistical analyses were performed using Fisher’s exact test in comparison to WT mosquitoes. IBM, infectious blood meal.